Nile Blue- Chitosan Composite as a new Electrochemical Sensor for Uric Acid on Glassy Carbon Electrode

Journal: Vol.8, No.1, Winter 2015 - Article 1   Pages :   Until 

Article Code:

Reza Sabzi: Urmia University - Department of Chemistry
Khalil Farhadi: Urmia University - Department of Chemistry
Elnaz MineiAnalytical Chemistry

Article's abstract:

Nile blue-Chitosan NB-CH as a new type of organic days-organic composite was made using Nile blue and chitosan. The NB-CH composite was made using a 0.005 M solution of Nile blue NB and a 7 solution of chitosan. To do this, a mixture of Nile blue and Chitosan with the ratio of 2:1 was prepared. The electrocatalytic oxidation of uric acid UA has been studied on glassy carbon electrode by NB-CH using electrochemical techniques. The NB-CH electrode showed one pair of peaks in 0.5 M KHSO4 as a supporting electrolyte. The catalytic currents are linearly related to UA concentrations in the ranges of 3×10-5 to 1.8×10-4 M and limit of detection 1.2 ×10-6 M with surface coverage of 2 ×10-8 mol cm-2.

Nile blue, Chitosan, Dye, Glassy carbon electrode, Uric acid

1. A. J. Cain, The use of Nile Blue in the examination of lipoids, Quart. J. Microscop. Sc., 88(1947), 383-92.#2. H. Ju, Y. Ye, Y. Zhu, Interaction between nile blue and immobilized single- or double-stranded DNA and its application in electrochemical recognition,. Electrochim. Acta, 50(2005), 1361-1367.#3. F. Shahidi, J. Synowiecki, Isolation and characterization of nutrients and value-added products from snow crab (Chionoecetes opilio) and shrimp (Pandalus borealis) processing discards, J. Agricul. Food Chem., 39(1991), 1527-1532.#4. I. Tsigos, A. Martinou, D. Kafetzopoulos, V. Bouriotis, Chitin deacetylases: new, versatile tools in Biotechnology, TIBTECH, 18(2000), 305-312.#5. P. Miretzkya, A. F. Cirelli, Hg(II) removal from water by chitosan and chitosan derivatives: A review. J. Hazard. Mater. 167(2009), 10–23.#6. S. L. Nishimura, O. Kohgo, K. Kurita, H. Kuzuhara, Chemospecific manipulations of a rigid polysaccharide: syntheses of novel chitosan derivatives with excellent solubility in common organic solvents by regioselective chemical modifications, Macromolecules, 24(1991), 4745-4748.#7. O. S. Amuda, A. A. Giwa, I. A. Bello, Removal of heavy metal from industrial wastewater using modified activated coconut shell carbon, Biochem. Eng. J., 36(2007), 174-181.#8. P. Sanguansri, M. A. Augustin, Nanoscale materials development a food industry perspective, Trends in Food Sci. Technol., 17(2006), 547-556.#9. S. M. Nomanbhay, K. Palanisamy, Removal of heavy metal from industrial wastewater using chitosan coated oil palm shell charcoal, Electron. J. Biotechnol., 8(2005), 43-53.#10. L. Dambies, C. Guimon, S. Yiacoumi, E. Guibal, Characterization of metal ion interactions with chitosan by X-ray photoelectron spectroscopy, Colloids Surf., 177(2001), 203-214.#11. A. R. Cestari, E. F. S. Vieira, A. G. P. dos Santos, J. A. Mota, V. P. de Almeida, Adsorption of anionic dyes on chitosan beads. 1. The influence of the chemical structures of dyes and temperature on the adsorption kinetics, J. Colloid Interface Sci., 280(2004), 380-386.#12. D. Han, T. Han, C. Shan, A. Ivaska, L. Niu, Simultaneous determination of ascorbic acid, dopamine and uric acid with chitosan-graphene modified electrode, Electroanalysis, 22(2010), 2001-2008.#13. M. Zhang, A. Smith, W. Gorski, Carbon nanotube-chitosan system for electrochemical sensing based on dehydrogenase, Enzymes. Anal. Chem., 76(2004), 5045-5050.#14. F. Ni, H. Feng, L. Gorton, T. M. Cotton, Electrochemical and SERS studies of chemically modified electrodes: Nile Blue A, a mediator for NADH oxidation, Langmuir, 6(1990), 66-73.#15. Z. Nazemi, E. Shams, M. K. Amini, Covalent modification of glassy carbon electrode by Nile blue: Preparation, electrochemistry and electrocatalysis, Electrochim. Acta, 55(2010), 7246-7253.#16. Z. Nasri, E. Shams, M. Ahmadi, A glucose biosensor based on direct attachment of in situ generated nile blue diazonium cations to the electrode surface, J. Electroanal. Chem., 703(2013), 146-152.#17. F. S. Saleh, L. Mao, T. Ohsaka, Development of a dehydrogenase-based glucose anode using a molecular assembly composed of nile blue and functionalized SWCNTs and its applications to a glucose sensor and glucose/O2 biofuel cell, Sens. Actuators B, 152(2011), 130-135.#18. A. J. Bard, L. R. Faulkner, Electrochemical methods. fundamentals and applications, Wiley, New York, 1980, 234-238.#19. M. Mazloum-Ardakani1, H. Beitollahi1, B. Ganjipour, H. Naeimi, Novel carbon nanotube paste electrode for simultaneous determination of norepinephrine, uric acid and D-Penicillamine, Int. J. Electrochem. Sci., 5(2010), 531-546.#20. L. Fernández, H. Carrero, Electrochemical evaluation of ferrocene carboxylic acids confined on surfactant–clay modified glassy carbon electrodes: oxidation of ascorbic acid and uric acid, Electrochim. Acta, 50(2005), 1233-1240.#21. Z. Galus, Fundamentals of Electrochemical analysis, Ellis Horwood press, New York 1976, 126-134.

Article's file
Page view: 3306
Article's download quantity : 288

Article System Login

Prof. Zahra Ranjbar
Dr. Farahnaz Nourmohammadian
Assistant Editor:
Dr. Mozhgan Hosseinnezhad
ISSN (online):
Institute for Color Science and Technology (ICST)

Quick Access
What is your opinion about the manuscript subscription system of PCCC website?
Website Statistics
Page view:3,307
Online Visitors:73